454 pyrosequencing-based rna-seq Search Results


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Publications containing applications of second generation sequencing in rubber tree transcriptome analysis
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Publications containing applications of second generation sequencing in rubber tree transcriptome analysis
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
Fosmid Based 40 Kb Mp Library Nxseq 40 Kb Mp Cloning Kit, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
Miseq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic <t>RNA-seq</t> samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1
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Image Search Results


Publications containing applications of second generation sequencing in rubber tree transcriptome analysis

Journal: BMC Research Notes

Article Title: RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis

doi: 10.1186/1756-0500-7-69

Figure Lengend Snippet: Publications containing applications of second generation sequencing in rubber tree transcriptome analysis

Article Snippet: Overall, the depth of sequencing reported in these papers was between 0.2-5 Gb per sample based on Illumina PE-RNA-Seq or 454 pyrosequencing platforms (Table ).

Techniques: Sequencing

Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic RNA-seq samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1

Journal: Genome Biology

Article Title: Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome

doi: 10.1186/s13059-019-1660-0

Figure Lengend Snippet: Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic RNA-seq samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, ). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10 −9 ). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined. For complete numerical details, see Additional file : Supplemental Note 2.4. Analyses are based on OGS v1.1

Article Snippet: Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis , Acyrthosiphon pisum ; Clec , Cimex lectularius ; Focc , Frankliniella occidentalis (thysanopteran outgroup); Ofas , Oncopeltus fasciatus ; Pven , Pachypsylla venusta ; n.d., not determined.

Techniques: RNA Sequencing Assay, Expressing